Journal: PLoS ONE

Article Title: Differential Expression Profile of Chicken Embryo Fibroblast DF-1 Cells Infected with Cell-Adapted Infectious Bursal Disease Virus

doi: 10.1371/journal.pone.0111771

Figure Lengend Snippet: The demultiplexed, trimmed sequence reads were mapped to IBDV reference genome comprising two segments. No IBDV sequences were identified in the mock-infected group and at 0hpi of the caIBDV-infected group. The sequence reads mapped to segment A showed up at 6hpi, with 20.01% coverage of the segment. While at 12hpi, both segment A and B sequences were mapped covering 63.87% and 56.17% of the respective genome segments. Shaded: segment A; solid: segment B.

Article Snippet: Cell-adapted IBDV (caIBDV) was generated with propagating IBDV vaccine strain D78 (VR-2041, ATCC) in secondary chicken embryonic fibroblast cells, followed by purification with CsCl gradient and 20% sucrose gradient as described previously [ ].

Techniques: Sequencing, Infection

Journal:

Article Title: Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2

doi:

Figure Lengend Snippet: Reactivities of various MAbs to different IBDV strains

Article Snippet: Many CEF-adapted vaccine strains, including D78 (Intervet), IBD Blend (Sanofi Inc.), Bio-Burs (Kee-Vet), Bio-Burs I (Kee-Vet), Bursa-vac (Sterwin), Bursine (Solvary Inc.), Bur-706 (Rhone Merieux), Univax (American Sci.

Techniques: Mutagenesis

Journal:

Article Title: Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2

doi:

Figure Lengend Snippet: Cytopathogenicity of recombinant virus on CEF cells. CEF cells (2 × 104 in each well) were mixed with 100 TCID50 of D78 or HK46-NT. At daily intervals (up to 5 days), 20 μl of MTS/PES solution was added to each well and the plates were incubated at 37°C for 2 h in a humidified chamber with 5% CO2. The plates were then measured at OD490. The background reading, measured from wells with culture medium and MTS/PES only, was subtracted from the data. Each value is the average of two independent experiments.

Article Snippet: Many CEF-adapted vaccine strains, including D78 (Intervet), IBD Blend (Sanofi Inc.), Bio-Burs (Kee-Vet), Bio-Burs I (Kee-Vet), Bursa-vac (Sterwin), Bursine (Solvary Inc.), Bur-706 (Rhone Merieux), Univax (American Sci.

Techniques: Recombinant, Incubation

Journal:

Article Title: Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2

doi:

Figure Lengend Snippet: Growth curve of the mutant virus. CEF cells were infected with 100 TCID50 of D78 or HK46-NT in 200 μl of culture medium. Virus harvested at daily intervals was then titered and expressed as TCID50 per milliliter. Each value is the average of two independent experiments.

Article Snippet: Many CEF-adapted vaccine strains, including D78 (Intervet), IBD Blend (Sanofi Inc.), Bio-Burs (Kee-Vet), Bio-Burs I (Kee-Vet), Bursa-vac (Sterwin), Bursine (Solvary Inc.), Bur-706 (Rhone Merieux), Univax (American Sci.

Techniques: Mutagenesis, Infection